Figure 9.

Inhibition of 13-HODE-G and 13-HODE-EA biosynthesis by 15-LO inhibitors in human eosinophils and neutrophils. Pre-warmed (37 °C) human (A,B) eosinophil suspensions (10⁶ cells/mL in HBSS containing 1.6 mM CaCl₂) or (C,D) neutrophil suspensions (5 × 10⁶ cells/mL in HBSS containing 1.6 mM CaCl₂) were pre-treated with DMSO, BLX-3887 (10 µM) or NDGA (10 µM) for 5 min before the addition of 3 μM (A,C) LEA or (B,D) 1-LG. Incubations were stopped 5 min after LEA or 1-LG treatment by the addition of one volume of cold (−20 °C) MeOH containing the internal standards. Samples then were processed for LC-MS/MS analyses as described in methods. Results are the mean (±SEM) of 4 independent experiments.