Figure 6.

Cur and 5-FU regulate proteins related to cell cycle in CRC cells. Cells were treated with Cur (30 μM for HT-29 cells; 10 μM for SW480 cells) and/or 5-FU (20 mg/L for HT-29 cells; 10 mg/L for SW480 cells) for 24 h. (A–I) Proteins were analyzed by Western blot and are displayed as a histogram in HT-29 cell lines. The untreated groups of HT-29/NC were used as the control group, which was set as 1; (J–R) Proteins were analyzed by Western blot and are displayed as a histogram in SW480 cell lines. The untreated groups of SW480/NC were used as the control group, which was set as 1. 5-FU: 5-Fluorouracil, Cur: curcumin. Data are expressed as means ± SD, n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001.