Figure A1.

Cur combined with 5-FU induces cell apoptosis in CRC cells. Cells were treated with Cur (30 μM and 35 μM for HT-29 cells; 15 μM and 20 μM for SW480 cells) and/or 5-FU (20 mg/L for HT-29 cells; 10 mg/L for SW480 cells) for 24 h. (A,C) Cell apoptosis was analyzed by flow cytometry and displayed as a histogram of HT-29 cells. (B,D) Cell apoptosis was analyzed by flow cytometry and displayed as a histogram of SW480 cells. 5-FU: 5-Fluorouracil, Cur: curcumin. Data are expressed as means ± SD, n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001.