Figure 4.

Bioassay of the purified Trichoderma asperellum (P1) active ingredient(s). (A) The purified active compound(s) in the growth medium of T. asperellum that inhibit M. maydis growth (described in Figure 3) were embedded in PDA at the indicated dosages. Thereafter, a plate M. maydis inhibition assay was performed for three days. Controls (on the left) are M. maydis grown on PDA and PDA embedded with P1 growth medium (crude) extracts. Vertical upper bars represent the standard error of the mean of four replicates. Levels not connected by the same letter (A–E) differ significantly from the other treatments in the same measure (p < 0.05, ANOVA). (B) Representative Petri dishes photograph of each treatment at the experiment’s end.