Figure 7.

Seeds phytotoxicity assay for the 6-Pentyl-α-pyrone (6-PP). (A) T. asperellum (P1)-secreted metabolites (growth medium) or commercial 6-PP compound was embedded in PDA at the indicated dosages and tested for M. maydis inhibition, as described in Figure 2. (B) Impact of growth media of P1 or commercial 6-PP compound on corn seed germination. In each Petri dish, five grains were seeded in 3.5 mL of tap water, secretion products (growth medium filtrate after 6 days P1 growth), or commercial 6-PP compound at various concentrations, v/v). Data are average after three days of incubation. Vertical upper bars represent the standard error. Levels not connected by the same letter (A–E) differ significantly from the other treatments in the same measure (p < 0.05, ANOVA).