The effects of administering rapamycin-preconditioned ADSCs on liver PI3K-AKT signaling pathway and NF-κB gene expression in cisplatin (Cis)-induced liver damage. (A) PI3K relative mRNA expression in the study groups. (B) AKT relative mRNA expression in the study groups. (C) NF-κB relative mRNA expression in the study groups. Cis, cisplatin (i.p. injection of 5 mg/kg in 0.9% saline) group; Cis + ADSCs, cisplatin group receiving ADSCs (one million cells, intravenous in tail vein); Cis + Rapa-ADSCs, cisplatin group receiving same number of ADSCs preconditioned with rapamycin (50 nM/L, 2 h prior to administration). Statistical significance, * vs. control group (p < 0.01), # vs. Cis group (p < 0.01) and $ vs. Cis+ADSCs group (p < 0.05).