FIG. 12.

Microinjection of mRNA encoding Oct-2_HD into one- or two-cell-stage zebra fish embryos rescues the developmental defects induced by GR_C460Y. The outcome at 24 h is displayed for one- or two-cell-stage embryos injected with 0.2 to 0.4 nl of RNA encoding GR_C460Y (600 μg/ml), saline alone, or RNAs encoding GR_C460Y and Oct-2_HD (1,200 μg/ml). The peptides encoded by the RNAs injected are listed at the left, followed by the total number of embryos injected (n) and the total number of independent injection series performed with each sample. To the right, the percentage of embryos failing to survive for 24 h following injection is indicated by dark gray bars (D), the percentage of embryos with malformations visible under the stereomicroscope is indicated by light gray bars (M), and the percentage of normal embryos is indicated by white bars (N). The error bars indicate the standard deviations for independent trials (for GR_C460Y and GR_C460Y plus Oct-2 compared to the controls, the P value was <0.001).