FIG. 2.

The GR DBD binds directly to Oct-2_HD. GST-tagged WT GR DBD and GR_L501P DBD (aa 407 to 550) containing PKA consensus phosphorylation sites were expressed in and purified to homogeneity from Escherichia coli and labeled with ³²P by use of PKA. Signals obtained with 10% of the ³²P-labeled GR and GR_L501P DBDs added to the binding assay are shown in lanes 1 and 5. The ³²P-labeled GR peptides were tested for binding to the POU domain and POU homeodomain of Oct-2 or to GST alone (lanes 2 to 4 and 6 to 8, respectively). MW, molecular weight (in thousands).