FIG. 4.
One-hybrid analysis of the L501P-sensitive interaction of the GR DBD with full-length Oct-2, hoxd4, and dlx2 in mammalian cells. The GR DBD, the DBD with an L501P substitution fused to the GAL4 DBD, or the GAL4 DBD alone was coexpressed with Oct-2, hoxd4, dlx2, or CREB in CHO cells. At 48 h after transfection, transcription from a reporter gene with an E1B minimal promoter and five GAL4 binding sites was assessed by a CAT assay. The data are expressed as the fold induction of CAT activity in the presence of GAL-GR DBDL501P (hatched bars) and GAL-GR DBD (solid bars) versus in the presence of GAL4 DBD (GALO). The error bars represent the standard error of the mean of three to five independent experiments performed in duplicate (P < 0.02).