FIG. 5.
The binding of full-length GR in nuclear extracts to full-length zebrafish dlx2 and hoxd4 is sensitive to GRL501P. (A) Binding of full-length, 35S-labeled, in vitro-translated dlx2 (lanes 2 and 3) and hoxd4 (lanes 6 and 7) to GR and GRL501P immunoprecipitated with c-myc antibody 9E10 from whole-cell extracts prepared from dexamethasone-treated, stably transfected murine SF-7 fibroblasts. Lanes 4 and 8 show binding to extracts prepared from untransfected SF-7 cells, while lanes 1 and 5 show 10% of the in vitro-translated homeodomain proteins added to the binding assay. (B) Western blot of the GRs immunoprecipitated from stably transfected SF-7 cells expressing WT GR and GRL501P and from untransfected SF-7 cells (lanes 9 to 11). Below each lane in panels A and B are the counts obtained following phosphorimager analysis.