FIG. 6.
Microinjection of mRNA encoding a GR peptide with the homeodomain binding surface into one- or two-cell-stage zebra fish embryos interferes with embryogenesis in an L501P-sensitive manner. The outcome at 24 h is displayed for one- or two-cell-stage embryos injected with 0.2 to 0.4 nl of RNAs (600 μg/ml) encoding GR mutant peptides with a GR DBD backbone (aa 407 to 556), an antisense transcript of the WT GR, or the MR DBD (aa 567 to 700) or with saline alone. The peptides encoded by the RNAs injected are listed at the left, followed by the total number of embryos injected (n) and the total number of independent injection series performed with each sample. To the right, the percentage of embryos failing to survive for 24 h following injection is indicated by dark gray bars, while the percentage of embryos with malformations visible under the stereomicroscope is indicated by light gray bars. The error bars indicate the standard deviations for independent trials (for GRC460Y and GRK489R compared to the controls, the P value was <0.001).