FIG. 8.
The expression of the GRC460Y peptide in zebra fish embryos disrupts the pattern of no tail expression during embryogenesis. In situ hybridization of whole-mount zebra fish embryos with a no tail antisense RNA probe at the indicated times after fertilization is shown. Embryos were injected at the one- or two-cell stage with saline (mock) (A, F, K, and P) or mRNAs encoding the GR DBD (aa 407 to 556) with C460Y (B to D, G to I, L to N, and Q) or C460Y and L501P (E, J, and O) mutations. Three examples of the hybridization patterns observed upon expression of the GRC460Y peptide at each time point are shown. Dorsal views are shown in panels A and C to O, while an animal pole view is shown in panel B and a lateral view of the tail is shown in panels P and Q. The arrow in panel Q indicates the secondary notochord axis. a, axial mesoderm; gr, germ ring; es, embryonic shield; n, notochord. Scale bar: A to O, 100 μm; P and Q, 70 μm.