Figure 2.

Effects of luteolin on the nucleus and mitochondria of GBM cells. (A) The A172 and U-373MG cells treated with luteolin (100 and 200 μM for 24 h) showed nuclear condensation and fragmentation. The nuclear morphology of the GBM cells was confirmed using Hoechst staining. (B) Mitochondrial membrane potential was determined by JC-1 staining (upper), and Bcl-2 and Bax expressions were determined using Western blot assay. The A172 and U-373MG cells were treated with 100 and 200 μM luteolin. In both cell lines, the JC aggregate (red fluorescence intensity) was reduced by luteolin. In the luteolin-treated cells, Bcl-2 was decreased and bax was increased in a dose-dependent manner.