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Induction of apoptosis by luteolin A172 and U-373MG cells. (A) Luteolin (200 μM) activates cleaved caspase-3 in both GBM cells, as confirmed by immunocytochemistry using confocal microscopy. (B) Luteolin (0–200 μM) treatment activated caspase-3 and caspase-7 and cleaved PARP. The protein expression levels were detected by Western blot assay. β-actin was used as internal control. (C) Apoptotic cell populations detect by Annexin V/PI staining in luteolin-treated GBM cells.
