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Inhibition of luteolin-induced autophagy further promotes cell death. A172 and U-373MG cells were pretreated for 1 h with 3MA (1 mM), an inhibitor of autophagy, followed by treatment with luteolin (50 µM) and then cultured for 24 h. The inhibitory effect of autophagy was confirmed by AO staining (A), MTT assay (B), and Western blot assay (C). β-actin was used as loading control. The results are expressed as mean ± SD.
