Table 1.

A list of the most commonly used in vitro assays for the determination of antioxidant activity (adapted from [50]).

Hydrogen atom transfer (HAT)-based assays	2,2-azino-bis (3-ethyl benzothiazoline-6-sulfonic acid) diammonium salt (ABTS+) radical scavenging [51] β-carotene bleaching [52] crocin bleaching [53] hydrogen peroxide (H2O2) scavenging [54] hydroxyl radical averting capacity (HORAC) [55] hydroxyl scavenging [56] inhibited oxygen uptake (IOU) [57] lipid peroxidation inhibition capacity (LPIC) [58] oxygen radical absorbance capacity (ORAC) [40] photochemiluminescence (PCL) [59] total radical trapping antioxidant parameter (TRAP) [41]
Electron transfer (ET)-based assays	1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging [46,47] cupric reducing antioxidant capacity (CUPRAC) [60] ferric reducing antioxidant power (FRAP) [44,45] ferric thiocyanate (FTC) [61] nitric oxide radical scavenging [62] N,N-dimethyl-p-phenylene diamine (DMPD) radical scavenging [63] peroxyl radical scavenging [64] potassium ferricyanide reducing power (PFRAP) [65] superoxide anion radical scavenging [66] thiobarbituric acid reactive substances (TBARS) [67] total phenolics content (TPC) using Folin-Ciocalteu reagent [42] trolox equivalence antioxidant capacity (TEAC) using ABTS [43]
Other in vitro methods	ascorbic acid content [68] cellular antioxidant activity (CAA) [69] metal chelating activity [70] scavenging of phosphomolybdenum [71] scavenging of xanthine oxidase [72]