Figure 5.

HLMVEC were incubated for 6 h in fresh RPMI1640 growth medium (control) in the absence (none) or in the presence of 10 nM SARS-CoV-2 spike protein supplemented with 2 µg/mL Polymixin B (S1) or in MDM conditioned medium (MDM-CM), obtained by incubating MDM for 16 h in the absence (none) or in the presence of 10 nM S1 and 2 µg/mL Polymyxin B. The expression of the indicated genes (left panels) was then measured by means of RT-qPCR, normalized for that of the housekeeping gene RPL15, and expressed relative to none in control cells (=1); data are means ± SEM of three experiments, each performed in duplicate. * p < 0.05 vs. none in control with “one-sample t-test”. The amount of the corresponding proteins (right panels) was assessed by means of Western Blot analysis (see Section 2); the representative blots shown were repeated twice and gave comparable results.