FIG. 5.

AID. (A) FL Xaxin fused to the GAL4 activation domain was cotransformed into S. cerevisiae with the constructs above fused to the GAL4 DNA binding domain. After growth on selective medium, colonies were assayed for expression of β-galactosidase to identify clones which harbored interacting proteins. FL Xaxin, ΔDIX, Y2H4, and AID constructs all interacted strongly with FL Xaxin. Y2H6 and Y2H7 had no significant interaction with FL Xaxin while retaining the ability to interact with GSK-3β (16). +, positive interaction; −, no interaction. (B) Myc-tagged FL Xaxin was coexpressed with HA-tagged FL Xaxin in embryos. Samples were immunoprecipitated (IP) with the anti-Myc antibody and immunoblotted with anti-HA antibodies. Top, immunoprecipitates; bottom, embryo lysates prior to immunoprecipitation.