FIG. 7.

ΔGID reverses dorsalization by ΔRGS. (A) Schematic of proposed complementation of ΔRGS by ΔGID utilizing the AID. The GID lacks the AID. (B) Axis duplication. ΔRGS and GID cause complete axis duplication, with eyes and cement glands present. Coexpression of ΔGID with ΔRGS (ΔRGS+ΔGID) reverses axis duplication, while coexpression of ΔGID with GID (GID+ΔGID) does not. ΔGID alone causes infrequent ectopic posterior axes. (C) Scoring for complete and partial secondary axes. Filled bars represent complete secondary axes (including eyes and cement glands); open bars represent partial secondary axis, including head but lacking eyes or cement gland. The few secondary axes in ΔGID-injected embryos showed posterior duplications without head formation. ΔGID did not affect the level of ΔRGS expression as detected by immunoblotting (data not shown).