Table 2.
Evaluated analytical columns for the LC-MS/MS method.
| Column | tR (DON) (min) | tR (D3G) (min) | tR (3ADON) (min) | tR (15ADON) (min) | FWHM (min) | RS (DON/D3G) | RS (3/15ADON) |
|---|---|---|---|---|---|---|---|
| Agilent Zorbax Eclipse Plus | 2.63 | 2.68 | 4.16 | 4.17 | 0.045 | −1.11 | −0.22 |
| Agilent Zorbax Extend-C18 | 2.15 | 2.26 | 3.85 | 3.86 | 0.077 | −1.43 | −0.13 |
| Agilent Poroshell EC-C18 | 5.30 | 5.14 | 7.30 | 7.16 | 0.057 | 2.82 | 2.47 |
| Agilent Zorbax SB C18 | 5.87 | 5.87 | 9.00 | 9.02 | 0.085 | 0.00 | −0.24 |
| Agilent Zorbax XDB-C18 | 4.75 | 4.52 | 6.58 | 6.46 | 0.060 | 3.83 | 2.00 |
| Daicel Chiralcel | 3.59 | 3.45 | 11.00 | 9.00 | 3.000 | 0.05 | 0.67 |
| Daicel Chiralpak | 2.29 | 2.06 | 9.29 | 9.08 | 0.143 | 1.60 | 1.47 |
| Phenomenex Kinetex C18 | 4.07 | 4.03 | 5.81 | 5.82 | 0.075 | 0.53 | −0.13 |
| Phenomenex Kinetex F5 | 4.26 | 4.15 | 6.33 | 6.23 | 0.067 | 1.65 | 1.50 |
| Sigma Discovery HS F5 | 6.14 | 5.95 | 9.88 | 9.68 | 0.122 | 1.56 | 1.64 |
| Thermo Hypersil Gold | 1.84 | 2.13 | 3.68 | 3.69 | 0.112 | −2.60 | −0.09 |
| Waters Acquity BEH C18 | 2.98 | 3.21 | 4.37 | 4.40 | 0.040 | −5.75 | −0.75 |
| Waters Acquity HSS T3 C18 | 5.20 | 4.93 | 7.17 | 7.02 | 0.052 | 5.23 | 2.90 |
tR retention time, FWHM full width half maximum, Rs resolution. A water–acetonitrile gradient (5% → 60% acetonitrile in 9 min) containing 2 mM ammonium acetate was used for separation. The flow rate was 0.2 or 0.4 mL/min, depending on the column length.