Figure 5.
No DMSO effect on P. larvae vegetative cells and spores. (A) No significant difference in growth of vegetative P. larvae in MYPGP liquid culture was found under anaerobic conditions in the presence of different concentrations of DMSO (2-way ANOVA, p = 0.87). Symbols indicate the mean ± SEM from three biological replicates with three technical replicates each. (B) No significant influence on germination of P. larvae spores was detected on MYPGP agar plates containing different concentrations of DMSO (2-way ANOVA, p = 0.58). Symbols indicate the mean ± SEM from three biological replicates with two- three technical replicates each. (Circle colors: white: negative control; with dot: 0.004% (v/v) DMSO; black-white circle: 0.04% (v/v) DMSO; black circle: 0.4% (v/v) DMSO).