Figure 1.

Visual representation of the micropropagation process conducted for Cannabis sativa in this study. (A) Stem cutting taken from a mother plant. Nodal segments were excised and used as starting material for micropropagation. (B) Surface-sterilized, 1-cm long nodal cutting inoculated on shoot initiation medium (one week since initiation). (C) Close-up of a micropropagated nodal segment recovering on recovery medium (one week since plating). (D) Nodal explants excised from four-week-old micropropagated plants and plated directly onto recovery medium (approximately four weeks since plating). White arrows denote axillary nodes located on mother plant cutting. Scale bar represents 1 mm.