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. 2021 Aug 28;10(9):1794. doi: 10.3390/plants10091794

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Survival and regrowth of Cannabis sativa Cultivar 2 explants plated on MS basal recovery medium and incubated under various light treatments. Donor plants were either grown under common tissue culture temperatures (24 ± 2 °C) or incubated under cold conditions (10 ± 2 °C) one week before explant excision. Survival and recovery were analyzed separately. Bars displaying significant differences are denoted by different lower-case letters (p < 0.05). AT: Ambient temperature. Test#1—Light treatment: darkness for 7 days, gradual light for 7 days, ambient light for 16 days. Test#2—Light treatment: darkness for 7 days, ambient light for 23 days.