Figure 4.

qPCR analysis of randomly selected ABC transporters in different developmental stages of Ae. aegypti mosquito. E = Eggs, L = Larvae (numbers indicate the instar of larvae L1 to L4), P = Pupae, M = Males and F = Females. All samples were collected from three biological replicates at each developmental stage, error bars indicate mean ± SD (n = 3). The relative mRNA levels were calculated as mentioned in Materials and Methods. The mRNA levels in eggs were considered as 1.0 in each analysis. Significant differences among relative mRNA levels are represented with different alphabets like “a, b, c”. The ‘a’ alphabet bearing bars have non-significant expression change to each other, while they have significant differences with bars bearing other alphabets (b & c).