Figure 6.

Comparative analysis of the microarray and qPCR expression data of a few representative ABC transporter genes (as mentioned on the x-axis) during DENV2 infection. The fold change in mRNA expression was calculated by ^ΔΔCt method as described in Materials and Methods, using the actin gene as an internal control. All samples were collected from three biological replicates of each treatment (DENV2 infected blood— v/s uninfected blood-fed) at specified time intervals. Error bars indicate mean ± SD (n = 3). D1, D2 and D7 indicate the days post-viral infection. The sequence of the primers used in qPCR is given in Supplementary Table S1.