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Coupling efficiency of the recombinant proteins. Comparison of the median fluorescence intensity (MFI) values between the singleplex and multiplex assays. The recombinant proteins present on the surface of the beads were detected using biotinylated anti-GST antibody and streptavidin-R-phycoerythrin. The reading was performed on the Bio-Plex 200 (Bio-Rad) instrument as described in “Methods”.
