FIG. 4.

Down regulation of cPKC by Gö6976 counteracts nuclear accumulation of the wild-type p53Val135 conformational species in S100B-MEF cells. (A) Western blot analysis of p53Val135 protein accumulation in clone J-β2p53 cells stimulated with 1 μM ionomycin in the absence (S100B-MEF) or in the presence of 1 μM Gö6976 (S100B-MEF-Gö6976). p53 was detected by a mixture of monoclonal antibodies PAb421 and PAb240. The asterisk indicates a cross-reacting protein that serves as internal loading control. (B) Microscopic analysis of PAb246 immunoreactivities in S100B-MEF clone J-β2p53 cells grown at 37.5°C and stimulated for 18 h with 1 μM ionomycin in the absence (Iono.) or in the presence (Iono. Gö6976) of 1 μM Gö6976. Left panels show DNA staining with Hoechst 33258.