Figure 3.

C57/BL6 mice were exposed to either sham surgery (Sham) or cecal ligation and puncture (CLP), and were treated with the AMPK activator 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR), (CLP+A) or vehicle (CLP). At 24 h, the animals were sacrificed and samples were obtained to assess outcomes. For more details on the experimental design please see the Supplementary Material. There was no difference in the bacterial colony forming units (CFU) between mice treated with AICAR or vehicle in the peritoneal fluid (A) or in blood (B). In addition, there were no differences in plasma levels of interleukin-6 (IL-6) (C). These results suggest that protection through AMPK activation is independent of resistance because there was no effect on bacterial burden, and therefore, must be operating through a Tolerance mechanism. * p value < 0.05 when compared to sham.