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. 2021 Sep 12;22(18):9853. doi: 10.3390/ijms22189853

Figure 6.

Figure 6

The effect of tangeretin on ANGPTL3 promoter activity. (a) HepG2 cells were cotransfected with an ANGPTL3 promoter–reporter plasmid (ANGPTL3 p(−980/+20)) and a Renilla control plasmid for 24 h. The cells were then treated with vehicle (0.1% DMSO) or tangeretin (20 and 40 μM) for 24 h. The luciferase activities were measured and normalized to their respective Renilla luciferase activities. The data represent the mean ± SD from three independent experiments. ** p < 0.01 indicates significant differences compared to the vehicle-treated cells. (b) Reporter plasmids containing serially deleted DNA fragments of the ANGPTL3 promoter. (c) HepG2 cells were cotransfected with serially deleted ANGPTL3 promoter–reporter plasmids and a Renilla plasmid for 24 h and then treated with vehicle or tangeretin (40 μM) for an additional 24 h. The luciferase activities were measured and normalized to the respective Renilla luciferase activity. The data represent the mean ± SD of three independent experiments. ** p < 0.01 indicates a significant difference compared to vehicle-treated ANGPTL3 p(−980/+20) plasmid-transfected cells. ## p < 0.01 indicates significant differences compared to the respective vehicle-treated cells. “ns” indicates no significance.