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. 2021 Aug 30;9(9):1119. doi: 10.3390/biomedicines9091119

Figure 5.

Figure 5

Inhibition of Nrf2 using siRNA and brusatol induced cell cycle arrest and promoted cell death in vitro: (A,B) cell cycle progression was measured using PI staining followed by FACS analysis of the stained cells. A significant increase in sub-G0-G1 cell population was detected in MCF-7 (A) and MDA-MB-468 (B) cells transfected with siRNAs targeting Nrf2. (C–E) Cell cycle using PI staining followed by analysis of stained cells using FACS (C) and apoptosis (D,E) were measured using AO/EB dual staining. Treatment with brusatol (0.5, 5.0, and 10.0 µM) arrested MDA-MB-468 cells (C) in G2/M phase and increased sub-G0-G1 cell population. A significant increase in the number of dead cells was observed in brusatol treated (19.5 nM to 1250 nM) MDA-MB-468 cells (D) p value = 0.0001) and MCF 7 cells (E) p value = 0.0001). One-way ANOVA test was used for the data analysis (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001). ns refers to non-significant.