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. 2021 Sep 14;22(18):9938. doi: 10.3390/ijms22189938

Figure 5.

Figure 5

Expression of exosomal CD9 and knocking-out ADAM17 expression in exosomes reduced the uptake of exosomes by Colo-320 recipient cells. (A) Flow cytometry quantitation of the uptake by Colo-320 recipient cells of fluorescently labelled exosomes produced by Colo-320, Colo-320/CD9 and Colo-320/ADAM17-KO cells, after 2 and 4 h or cell−exosome co-incubation. (B) Confocal microscopy images showing the uptake by Colo-320 recipient cells of the different exosomes as in (A). The bar graphs show the quantification of exosomal uptake by counting the particles internalized by at least 80 cells for each condition in three separate experiments. Statistical analysis performed was one-way ANOVA. * p < 0.05, ** p < 0.01 *** p < 0.001.