FIG. 4.

Transient transfection of BALB/MK2 keratinocytes with pcDNA3-C/EBPβ inhibits growth as indicated by the number of BrdU-positive S-phase cells. Twenty-four hours following transfection of BALB/MK2 keratinocytes with C/EBPβ or empty vector control, transfected keratinocytes were selected for 24 h with G418, and the number of S-phase BrdU-positive cells was determined at 0, 24, and 48 h post-G418 removal. The number of S-phase BrdU-positive cells was determined per 1,000 cells, and the results are expressed as the number of S-phase BrdU-positive cells per 1,000 cells × 100.