FIG. 8.

C/EBPβ-deficient mice demonstrate alterations in epidermal K1 and K10 expression. Whole-cell epidermal lysates were prepared from the epidermis of adult wild-type and C/EBPβ-deficient female mice. (A) Western blot analysis was conducted with specific antisera as indicated. Epidermal lysates from wild-type and C/EBPβ-deficient mice are represented by +/+ and −/−, respectively, and each lane contains protein from a different mouse. (B) Densitometric analysis was conducted on Western blot autoradiographs in which wild-type and C/EBPβ-deficient extracts were run on the same gel. Results are expressed as the mean ± standard deviation of six mice/group. K1 and K10 levels in the C/EBPβ-deficient mice were significantly different from the wild-type levels (P < 0.05, two-tailed Student’s t test).