FIG. 9.

Altered expression of K1 and K10 in attached, spontaneously detached, and suspension-cultured primary keratinocytes from C/EBPβ-deficient mice. (A) Primary newborn keratinocytes were maintained in low-calcium medium. At day 6 after plating, attached cells (A), spontaneously detached cells (D), and 16-h suspension-cultured cells (S) (see text) were collected; whole-cell lysates were prepared; and Western blot analysis was conducted as indicated. Lysates from wild-type and C/EBPβ-deficient samples are represented by +/+ and −/−, respectively. (B and C) Primary newborn keratinocytes were maintained in low-calcium medium. At day 7 after plating, attached cells (B) and 16-h suspension-cultured cells (C) (see text) were collected; RNA was isolated; and Northern blot analysis for K1 was conducted.