TABLE 1.
Interaction of NRIF3 with nuclear receptors in yeasta
| Bait | Prey | β-Galactosidase activity
|
||
|---|---|---|---|---|
| Without ligand | With ligand | Fold stimulation | ||
| LexA | NRIF3-B42 | 2.3 | 1.9 | 0.8 |
| LexA-TR | NRIF3-B42 | 1.8 | 125 | 69 |
| LexA-RAR | NRIF3-B42 | 0.1 | 0.1 | 1 |
| LexA-RXR | NRIF3-B42 | 0.2 | 63 | 315 |
| LexA-GR | NRIF3-B42 | 0.8 | 0.6 | 0.8 |
a
The LacZ reporter activities were determined for yeast strains harboring the indicated bait and prey plasmids in the presence or absence of cognate ligands as described in Materials and Methods. See the text for detailed explanations.