FIG. 5.

Effect of PI3-ki inhibitors on survival of 32D cells. (A) Cell survival. 32D/GR15 and 32D/IRS1/IR cells were incubated in medium with FBS only or with FBS supplemented with the respective growth factors (GF) (IGF-1 for 32D/GR15, insulin for 32D/IRS1/IR cells, or IL-3), plus or minus the indicated concentrations of LY294002, an inhibitor of PI3-ki. The numbers of surviving cells were determined at 24 h after IL-3 withdrawal and addition of the growth factors and the inhibitor. WT, wild type. (B) Phosphorylation (upper panel) and amounts (lower panel) of Akt/PKB in the same cell lines and with different treatments. The presence (+) or absence (−) of LY294002 (10 μM) is indicated. P-AKT, phosphorylated Akt. (C) BAD phosphorylation under the same conditions.