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Presence of mitRaf in 32D/GR15 and 32D/GR35 cells. Mitochondrial lysates were prepared as described in Materials and Methods, and the same amounts of protein were blotted for Raf-1 (upper panel). The amounts of mitochondrial proteins in each lane were monitored with an antibody to cytochrome oxidase (COX IV) (lower panel). I, IGF-1.
