FIG. 9.

Effect of mitRaf on cell survival. (A) 32D and 32D/GR35 cells were stably transfected with constitutively activated mutant Raf that localizes to the mitochondria. Transduction and selection of cell lines are described in Materials and Methods. Survival was determined at 48 h after IL-3 withdrawal, under the usual conditions. (B) Detection of mitRaf in the various cell lines. Detection was carried out with an anti-Raf1 antibody (Santa Cruz). The size of the mutant Raf is different from that of wild-type Raf-1, which is visible above the mutant Raf.