FIG. 2.
Molecular size of irreversibly inactivated ODC. ODC (mixture of purified rat ODC and metabolically labeled 35S-ODC from FM3A mouse cells) was incubated for 60 min at 37°C in an inactivation/degradation reaction mixture containing an extract of HTC cells that had been treated with Z-LLL-CHO. The decreases in ODC activity and ODC protein during incubation were 80 and 6%, respectively. Aliquots of the reaction mixture were subjected to SDS-PAGE and analyzed by autoradiography. Relative intensities of ODC bands determined with an image analyzer (Fujix BAS2000) are shown below each lane.