Figure 3.

Evaluation of a stirrer-based kinetic pump on the kinetic pump integrated microfluidic plate (KIM-plate). (A) Comparison of chemical transportation with/without stirrer bar rotation of the kinetic pump in the KIM-plate. The red dye diffused from chamber A to chamber B by the rotation of the kinetic pump. (B) Time-dependent change in the fluorescence intensity ratio (chamber B/chamber A) of Lucifer yellow applied into chamber A. Transportation was accelerated by increasing the rotation speed of the stirrer bar. Each datum represents the mean ± SD (n = 7–13). (C) Photographs of PXB cells in static/fluidic conditions. No difference in PXB cell number or morphology was observed between the nonperfusion and perfusion cultures.