Figure 1.

Luminex xMAP^® MIA format. (a) Polystyrene beads are internally colored with two different fluorescent dyes: red and infrared, with up to 100 distinct bead regions generated by using different concentrations of red and infrared dyes. Each bead region, bound to a different capture Ab, detects its specific analyte, followed by the binding of a biotinylated detection Ab and streptavidin-conjugated phycoerythrin (reporter dye). (b) The beads are identified individually in a rapidly flowing fluid stream that passes by two laser beams: red classification laser (635 nm) or LED reveals the color code of the bead region, and green reporter laser (532 nm) or LED determines the analyte concentration by measuring the reporter fluorescence intensity [13]. Reproduced with permission from Elsevier B.V. [13].