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Immunohistochemical staining of human dental pulp stem cells subjected to 14 d of mechanical vibrations. Collagen type I was strongly detected at 45 Hz and 100 Hz, and collagen type III was strongly detected at 45 Hz. Additionally, osteopontin (OP) and osteonectin (ON) were strongly detected at 45 Hz and 100 Hz. HE: Hematoxylin-eosin staining. Original magnification; HE: 100×, Col I and Col III: 200×, OP and ON: 400×, Scale bar; HE: 200 μm, Col I and Col III: 100 μm, OP and ON: 50 μm.
