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. 2021 Sep 7;9(9):997. doi: 10.3390/vaccines9090997

Figure 3.

Figure 3

Restriction enzyme analysis (REA) on IS1311-positive PCR products obtained from sheep-milk samples. The first and last lanes are 100-bp marker (MW); second lane is MAP standard strain 1515 (MAP-C). The remaining lanes belong to IS1311-targeted amplicons from sheep-milk samples that underwent REA analysis. Samples “13B, 20B, 31B, and 33B” at the BTM level and “7V, 62V, 72V, 76V, 83V, and 93V” at the individual level (MIF) are S-type while samples “6B, 10B, 14B, 23B, 28B, and 79B” at the BTM level and “65V” at MIF level are C-type, bison type samples “18B, 19B, and 29B” at the BTM level and “64V” at MIF level were also observed. Sample 12B yielded an unusual pattern not seen before (B and V letters by the numbers are indicating BTM and individual milk sample respectively).