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. 2021 Sep 2;9(9):1139. doi: 10.3390/biomedicines9091139

Figure 5.

Figure 5

The glycolytic side-product MGO modulates HIF-1α target genes related to cellular glucose metabolism. Western blot analysis of the glycolytic enzymes HK2 (A), PKM2 (B) and LDHA (C), and of the inhibitor of pyruvate dehydrogenase activity PDK1 (D) in total cell extracts from HUVEC treated (or untreated, Ctr = control) with the reactive dicarbonyl compound MGO (200 µM) for 48 h, silenced for HIF-1α (si-HIF-1α), or in the presence of the carbonyl trapping agent Car (20 mM), and relative band densitometry analysis from three separate experiments. mRNA levels of the mitochondrial biogenesis marker PGC-1α (E) in HUVEC exposed to MGO for 48 h, silenced for HIF-1α, or treated with Car; each dot represents the mean of two technical replicates of 5 wells per condition. Representative IF (F, scale bar 20 µm) for MT-CO1 in HUVEC treated or untreated (Ctr) with MGO for 48 h, in the presence or absence of Car. Bars represent mean ± SEM. Post hoc multiple comparison: *** p < 0.001 or * p < 0.05 vs. Ctr; ††† p < 0.001, †† p < 0.01 or † p < 0.05 vs. MGO.