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JIP1 and JIP2 enhance JNK activation by MLK3. Epitope-tagged (HA) JNK1α1 (A), JNK2α2 (B), and JNK3α2 (C) were each expressed alone or coexpressed with JIP1 and/or JIP2. JNK activity was examined by using c-Jun as a substrate. The effect of coexpression with MLK3 was examined. The amounts of JIP, MLK, and JNK expressed were examined by immunoblot analysis.
