Principal component analysis (PCA) showing the variability of the antioxidant and anti-enzymatic activity of comfrey roots depending on the storage period and geographical region. (A). Eigenvalues and percentage of explained variance of each principal component (PC). (B). Loading plots displaying the relationship between the biological activities and the three significant PCs. (C). Score plots showing the distribution of the samples in the three two-dimensional plans obtained from the three significant PCs. ABTS, 2,2′-azino-bis(3-ethylbenzothiazoline) 6-sulfonic acid; AChE, acetylcholinesterase; BChE, butyrylcholinesterase; CUPRAC, cupric ion reducing antioxidant capacity; DPPH, 1,1-diphenyl-2-picrylhydrazyl; FRAP, ferric ion reducing antioxidant power; MCA, metal chelating activity; PDA, phosphomolybdenum activity.