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. 2021 Sep 7;13(9):1415. doi: 10.3390/pharmaceutics13091415

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cytotoxicity assay of bare and CS-coated CBD/PLGA NPs against T24 and SV-HUC-1 cells. The viabilities of T24 (A,B) and SV-HUC-1 (E,F) cells were measured by incubating with CBD dissolved in medium and CBD dissolved in DMSO for 48 and 72 h. The viabilities of T24 (C,D) and SV-HUC-1 (G,H) cells were measured by incubating with Blank PLGA NPs, CBD/PLGA NPs and CS-CBD/PLGA NPs for 48 and 72 h. Data are expressed as mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the control.