mRNA expression of Cu regulatory proteins in control rats. Expressions of mRNA encoding Cu regulatory proteins were quantified by qPCR. (A). ∆∆Ct values of Cu transporters Ctr1 and Dmt1 in the control CP and OB. (B). ∆∆Ct values of metallothioneins Mt1a, Mt2a, and Mt3 in the control SVZ and OB. Data represents mean ± SEM, n = 6–7/group. aaa: p < 0.001, as compared with the Ct-CP; bbb: p < 0.001, as compared with the correspondent Ctr1 ∆∆Ct values of the same tissue; ccc: p < 0.001, as compared with the Ct-SVZ; ddd: p < 0.001, as compared with the correspondent Mt1a ∆∆Ct values of the same tissue. SVZ: subventricular zone; OB: olfactory bulb.