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. 2021 Aug 25;10(9):2187. doi: 10.3390/cells10092187

Figure 2.

Figure 2

Developmental exposure to CPF and ETU modulates THs levels and metabolism in mouse testis. (A,B) Testicular (t)FT4 and (t)FT3 levels were measured by ELISA in adult testes from CTRL and exposed males (N° = 3 testis/group), as described in M&M section. (C–G) Levels of the mRNAs of enzymes involved in THs metabolism (Dio1, Dio2, Dio3) and signaling (Thra, Spot14) in testes of mice exposed to pesticides and CTRL. RTqPCR tests were performed on three biological samples (n = 3 testis/group). Data are reported as fold change values calculated as a ratio between average relative gene expression in treated and control testes, after normalization on β-actin mRNA. (H,H’,I,I’) Western blot analysis showing the level of DIO3 protein following ETU and CPF treatment (N°3 testis/group). Data were obtained normalizing using B-ACTIN. Data analysis was conducted as described in M&M. One-way ANOVA (post hoc test: Dunnett’s) was used. * p < 0.05, ** p < 0.01, *** p < 0.001. Mean and standard deviation are reported.