Figure 2.

Parallelized perfusion setup: (A) Scheme of gravitation-driven flow. Medium reservoirs (V ≈ 1 mL) were placed on the channel inlet and outlet. Tubes reaching into the reservoirs in connection with micro-peristaltic pumps that operated intermittently were used to move perfused medium from the outlet reservoir back to the inlet reservoir, thus maintaining the level difference, Δh, driving unidirectional medium flow. (B) Chip in fixture with medium reservoirs attached on fluid ports of MFC. (C) Perfusion setup with 10 channel microfluidic pumps mounted on top of the chip fixture to yield an easy-to-use and incubator compatible perfusion system.